
Louis Pasteur's experiment with the swan-neck flask debunked spontaneous generation, confirming life arises from existing life, not nonliving matter.
Louis Pasteur disproved the theory of spontaneous generation using boiled beef broth and a flask with an S-shaped tubing. He demonstrated that life only comes from life through his famous swan-neck flask experiment, where he showed that sterilized broth remained free of contamination until exposed to air carrying microbes, proving that cells come from preexisting cells, not nonliving matter.
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