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(a) Given that the path length of the cuvette is 1 cm, what is the extinction coefficient of the 0.020 mM Yellow 5 dye at lambda max?(b) Given the data in the table below, what is the concentration of the diluted solution?Stock solution concentration 0.075 MStock solution absorbance 1.84Diluted solution absorbance 0.78

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Respuesta :

Answer:

(a). Kindly check the explanation section.

(b). 24.5 M^-1 cm^-

(c). 0.0318 M

Explanation:

So, we are given the following data parameters which is going to aid us in solving the above Question;

The path length = 1cm, extinction coefficient = unknown, absorbance is unknown and concentration= 0.02mM.

Using the formula below; we can determine the extinction coefficient as;

Absorbance = extinction coefficient × concentration × path length. -----------(1).

(a). Since, the absorbance is not given in the Question it won't be possible to determine the value of the extinction coefficient. Thus, say the value of absorbance = A.

Then, extinction coefficient = A/ 0.02 × 1 cm.

(b). Making use of the formula above, the extinction coefficient can be Determine as follows;

extinction coefficient = 1.84/0.075 × 1 = 24.5 M^-1 cm^-1.

(c). The concentration can be Determine by also making use of the formula above and making the concentration the subject of the formula;

Concentration = absorbance/ extinction coefficient × path length.

Concentration = 0.78/24.5 M^-1 cm^-1. × 1cm = 0.0318 M.